Fig. 1.
Induction of a protective immune response against tumor challenge by intratumoral injection of α-gal glycolipids. Primary B16 tumors received three weekly injections of 1 mg α-gal glycolipids (a), or one injection of ethanol (b). One day after last injection of α-gal glycolipids, or 3 weeks after ethanol injection, mice were challenged with 0.5 × 106 B16 cells in the contra-lateral left flank and tumor growth in the left flank monitored. Note that ten of 15 mice injected with α-gal glycolipids (a) displayed no tumor development in the left flank (one curve labeled with filled circle for all ten mice). Tumors in the remaining five mice are presented as individual curves each labeled with open circle. Mice with tumors injected with ethanol (b), all developed tumors in the left flank (n = 5)