Fig. 7.

Insertion of α-gal glycolipids into the cell membranes of human melanoma cells (SK-N-MC pLXSN cell line). a Binding of the monoclonal anti-Gal Ab M86 to untreated melanoma cells (thin line histogram), or cells incubated for 2 h at 37°C with 0.1 or 1 mg/ml α-gal glycolipids (dashed line and thick line histograms, respectively). b Complement dependent cytolysis of the human melanoma cells with inserted α-gal glycolipids following 30 min incubation at 37°C with human serum. Open circle cells incubated with 1 mg/ml α-gal glycolipids; open square 0.1 mg/ml; open triangle no α-gal glycolipids