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LAD2 cells were stimulated through the FcεRI for 0, 30 sec, 1 and 3 min, lysed and immunoprecipitated with anti-CD84 mAb. Membranes were blotted with anti-phosphotyrosine Ab and total CD84 levels were analyzed by western blot. Immunoprecipitation with an isotype control (IC) was also loaded.
