Fig. 7.

V protein expression counteracts the HCV-inhibitory effects of added type I and type III interferons in HFLC. Four days posttransduction with lentiviral vectors, HFLC (ABR-1108; 19 weeks gestation) were incubated with 1 × 10⁶ TCID₅₀ per well JC1G reporter virus for 5 hours. Cells were washed three times then refed with HDM containing serial 10-fold dilutions of IFN-β, IL-28A, or IL-29. The culture medium was aspirated and replaced every 2 days. Cytokine treatment was discontinued after the second day of culture. (A) JC1G reporter virus-derived luciferase activity detected in culture supernatants during the first 6 days of culture (mean and SD of two cultures). RLU, relative light units. (B) Levels of infectious HCV detected in cell culture supernatants on day 14 of culture (mean and SD of two assays on two cultures). TCID₅₀, tissue culture infectious doses; Fluc, firefly luciferase; PIV5 V, parainfluenza virus 5 V protein; MV V, measles virus V protein.