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. Author manuscript; available in PMC: 2011 Dec 7.
Published in final edited form as: Mech Dev. 2010 Aug 31;127(9-12):472–484. doi: 10.1016/j.mod.2010.08.005

Figure 3. FN and integrin α5 are required for the presence of normal numbers of CNCCs in branchial arches at E9.5.

Figure 3

A. Quantification of CNCCs located outside of the neural tube in 2 control (C1 and C2, closed bars) and 3 FN-null (N1–N3, open bars) embryos. Serial sections caudal to the otic vesicle were analyzed. C1 and C2: three sections for each embryo through 30 – 50 μm of tissue were counted; N1: nine step sections, 5 μm in thickness, through 90 μm of tissue were analyzed; N2: six sections through 60 μm of tissue were analyzed; N3: seven sections through 70 μm of tissue were analyzed. B. Quantification of TFAP2α+ cells in embryonic mesenchyme at each branchial arch level in control (closed bars) and FN-null (open bars) embryos at E9.5. Total of 17 sections from FN-null (spanning about 200 μm of tissue and 13 sections from control embryo (covering approximately 150 μm of tissue) were analyzed. p< 0.001 for all null vs control comparisons. BA – branchial arch. Number of cells were counted in each section and averaged among sections. C, D. Quantification of CNCCs outside the neural tube in Pax3Cre/+ (C) and Wnt1-Cre (D) labeled control and integrin α5-null embryos. Serial sections caudal to the otic vesicle were analyzed. C. Pax3Cre/+ fate mapping. Average number of CNCCs per section is plotted. C1: 9 step sections and C2: 5 step sections were analyzed; N1: 31 serial sections spanning about 300 μm of tissue were analyzed, N2: 13 step sections, N3: 14 step sections were analyzed. D. Wnt1-Cre fate mapping. Average number of CNCCs per section is plotted. C1: 10 sections and N1: 9 sections were examined.