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. Author manuscript; available in PMC: 2012 Dec 2.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Nov 3;415(4):662–668. doi: 10.1016/j.bbrc.2011.10.132

Fig. 2. Mutational analysis of the mouse MMP10 gene promoter region.

Fig. 2

(a–b) The structures of a series of MMP10-promoter luciferase reporter plasmids are shown schematically (left) and their relative luciferase activities are shown to the right of each construct. Numbers indicate the location of E-boxes relative to the transcription start site. MMP10 reporter constructs and pCDNA3-CHF1 were transfected into 293T cells. Results are representative of experiments done in triplicate. (c) Sequence analysis of the 2.5 kb upstream promoter sequence of the mouse MMP10 gene for potential regulatory elements with TFSEARCH (version 1.3, 1995, Kyoto University, Japan) and MotifMogul (version 1.0, November 2008, Institute of Systems Biology). E-boxes are marked in boxes. Putative recognition sites for other transcription factors are underlined and labeled.