Table 1. Primers used for the PCR amplification of the BEST1 gene.
Exon | Sense primer (5′-3′) | Antisense primer (5′-3′) | Annealing temperature (°C) |
---|---|---|---|
1 |
ACCCAACACCCTCCAAGAA |
AGTCCACCTGGGGCACCTT |
60 |
2 |
CCCTACAAACCCCCAATCG |
CCAGCCACATCCTTCCCAG |
60 |
3 |
AGTCTCAGCCATCTCCTCGC |
TGGCCTGTCTGGAGCCTG |
60 |
4 |
AGAAAGCTGGAGGAGCCGA |
TTCACCCATCTTCCATTCCT |
58 |
5 |
GCCATCCCTTCTGCAGGTT |
GCGGCAGCCCTGTCTGTAC |
62 |
6 |
GGGGCAGGTGGTGTTCAGA |
GGCAGCCTCACCAGCCTAG |
60 |
7 |
TGATTTCAGGGTTCCCACCTAG |
CATCCTCGTCTCAGGCAGCT |
65 |
8 |
AGGGTTTACAGAGCCTCACCTG |
CACTTTGGGGAAGGTCCATG |
60 |
9 |
CCTCCAAGTCATCAGGCACATA |
CTAGGCAGACCCCTGCACTAG |
65 |
40817 |
ACTGGCTCAGCCCTGCATC |
GTGGGGCACTGTAGTAGCCTG |
60 |
40818 |
CCTTCAAGTCTGCCCCACTG |
TGCAGTGCCCCTGGTTC |
60 |
11 | GGTACCTTCCATACTTATGCTG | AGGTCTTGGGATGAATCAGA | 60 |
For each exon the forward primer, the reverse primer, and the annealing temperature are indicated.