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. 2011 Nov 24;17:3078–3087.

Table 1. Primers used for the PCR amplification of the BEST1 gene.

Exon Sense primer (5′-3′) Antisense primer (5′-3′) Annealing temperature (°C)
1
ACCCAACACCCTCCAAGAA
AGTCCACCTGGGGCACCTT
60
2
CCCTACAAACCCCCAATCG
CCAGCCACATCCTTCCCAG
60
3
AGTCTCAGCCATCTCCTCGC
TGGCCTGTCTGGAGCCTG
60
4
AGAAAGCTGGAGGAGCCGA
TTCACCCATCTTCCATTCCT
58
5
GCCATCCCTTCTGCAGGTT
GCGGCAGCCCTGTCTGTAC
62
6
GGGGCAGGTGGTGTTCAGA
GGCAGCCTCACCAGCCTAG
60
7
TGATTTCAGGGTTCCCACCTAG
CATCCTCGTCTCAGGCAGCT
65
8
AGGGTTTACAGAGCCTCACCTG
CACTTTGGGGAAGGTCCATG
60
9
CCTCCAAGTCATCAGGCACATA
CTAGGCAGACCCCTGCACTAG
65
40817
ACTGGCTCAGCCCTGCATC
GTGGGGCACTGTAGTAGCCTG
60
40818
CCTTCAAGTCTGCCCCACTG
TGCAGTGCCCCTGGTTC
60
11 GGTACCTTCCATACTTATGCTG AGGTCTTGGGATGAATCAGA 60

For each exon the forward primer, the reverse primer, and the annealing temperature are indicated.