Table 6. Top 25 highly expressed transcripts in wild-type retina based on RNA-seq data.
| Transcript ID | Gene ID | Gene name | WT FPKM | Nrl−/− FPKM |
|---|---|---|---|---|
|
NM_145383 |
Rho |
Rhodopsin |
8135.4 |
1.7 |
|
NM_008140 |
Gnat1 |
Guanine nucleotide binding protein γ transducing activity polypeptide 1 |
4011.7 |
2 |
|
NM_008938 |
Prph2 |
Peripherin 2 |
1448.2 |
367.1 |
|
NM_012065 |
Pde6g |
Phosphodiesterase 6G cGMP-specific rod γ |
1269.5 |
552.6 |
|
NM_009073 |
Rom1 |
Retinal outer segment membrane protein 1 |
948.8 |
229.1 |
|
NM_015745 |
Rbp3 |
Retinol binding protein 3 |
885.3 |
1024 |
|
NM_009118 |
Sag |
S-antigen retina and pineal gland |
765.4 |
548.7 |
|
NM_011676 |
Unc119 |
Unc-119 homolog |
719.1 |
407.3 |
|
NM_024458 |
Pdc |
Phosducin |
689.8 |
302.3 |
|
NM_009038 |
Rcvrn |
Recoverin |
643.6 |
288 |
|
NM_011099 |
Pkm2 |
Pyruvate kinase muscle |
604.7 |
467.9 |
|
NM_001159730 |
Pdc |
Phosducin |
580 |
250.7 |
|
NM_146079 |
Guca1b |
Guanylate cyclase activator 1B |
552.6 |
68.1 |
|
NM_008131 |
Glul |
Glutamate-ammonia ligase |
545 |
530.1 |
|
NM_001136074 |
Nrl |
Neural retina leucine zipper |
545 |
1.9 |
|
NM_001160017 |
Gnb1 |
Guanine nucleotide binding protein β polypeptide 1 |
487.8 |
24.4 |
|
NM_011428 |
Snap25 |
Synaptosomal-associated protein 25 kDa |
471.1 |
576 |
|
NM_146086 |
Pde6a |
Rod photoreceptor cGMP phosphodiesterase a subunit |
433.5 |
35.8 |
|
NM_026358 |
4930583H14Rik |
Unknown |
407.3 |
362 |
|
NM_013415 |
Atp1b2 |
ATPase Na+K+ transporting β 2 polypeptide |
369.6 |
608.9 |
|
NM_144921 |
Atp1a3 |
Α 3 subunit of Na+K+ ATPase |
367.1 |
286 |
|
NM_008806 |
Pde6b |
Phosphodiesterase 6B cGMP-specific rod β |
364.6 |
16.2 |
|
NM_001160016 |
Gnb1 |
Guanine nucleotide binding protein β polypeptide 1 |
352.1 |
18.1 |
| |
(isoform 2) |
|
|
|
|
NM_008142 |
Gnb1 |
Guanine nucleotide binding protein β polypeptide 1 |
349.7 |
17.9 |
| |
(isoform 1) |
|
|
|
| NM_010314 | Gngt1 | Guanine nucleotide binding protein g transducing activity polypeptide 1 | 340.1 | 203.7 |
As photoreceptors constitute almost 70% of cells in P21 WT and Nrl−/− retina, the high expressed genes (in bold) likely encode proteins associated with general photoreceptor function/metabolism. WT=wild type