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Aaptamine induced p21 expression in K562 cells. The cells were incubated with 100 μM of aaptamine for the indicated times. Cell lysates were prepared and applied to 4–20% SDS-Polyacrylamide gel electrophoresis (SDS PAGE). After being transferred to polyvinylidene difluoride (PVDF) membrane, the blots were exposed to anti-Cip1/WAF-1/p21 or anti-β-actin, and then to anti-mouse horseradish peroxidase (HRP) conjugated IgG antibody.
