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Activation of p21 promoter in K562 cells. K562 cells were transfected with the human p21 promoter-luciferase fusion plasmid (pWWP), followed by treatment with various concentrations of aaptamine for 24 h, and then analyzed by luciferase assay. Fold increase of luciferase activity by aaptamine was calculated in comparison with the luciferase activity of the untreated cells. The result represents three independent experiments.
