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. 2011 Nov 7;12(11):7554–7568. doi: 10.3390/ijms12117554

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Characterization of 3-gene iPSCs overexpressing PGC-1α. (A) Upper: colonies of 3-gene iPSCs positive for alkaline phosphatase. Lower: differentiation of 3-gene iPSCs into ecto- (left), meso- (middle), and endo- (right) dermal lineages; (B) The iPSC colonies were positive for Oct-4 and Nanog; (C) Three-genes iPSCs were visualized using phase contrast microscopy; and (D) fluorescence microscopy 5 days after adenoviral infection; (E) RT-PCR analysis of PGC-1α expression in 3-gene iPSCs infected with the adenovirus expressing PGC-1α at 5 and 10 days post-transduction; (F) Relative changes in mitochondrial mass; (G) Relative changes in ROS production; (H) Relative oxygen consumption.