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. 2011 Nov 9;12(11):7732–7747. doi: 10.3390/ijms12117732

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Gene expression stability analyzed by the geNorm software in control sheep. (A) Average expression stability measure (M) of control genes during stepwise exclusion of genes with relatively higher variable expression among the samples; (B) Determination of the optimal number of control genes for normalization calculated on the basis of the pairwise variation (V) analysis; V values under 0.15 threshold indicate no need to include further reference genes for calculation of a reliable normalization factor.