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. 2011 Nov 9;12(11):7732–7747. doi: 10.3390/ijms12117732

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Gene expression stability analyzed by the geNorm software in combined control and disease-stressed sheep. (A) Average expression stability measure (M) of control genes during stepwise exclusion of genes with relatively higher variable expression among the samples; (B) Determination of the optimal number of control genes for normalization calculated on the basis of the pairwise variation (V) analysis; V values under 0.15 threshold indicate no need to include further reference genes for calculation of a reliable normalization factor.