Table 1.
Candidate reference genes for normalization of qRT-PCR ranked according to their expression stability by the geNorm applet.
| Control sheep (n = 18) | Disease-stressed sheep (n = 10) | Combined groups (n = 10 + 10) | |||
|---|---|---|---|---|---|
| Gene symbol | Stability value (M) | Gene symbol | Stability value (M) | Gene symbol | Stability value (M) |
| SDHA | 0.316 | YWHAZ | 0.624 | SDHA | 0.591 |
| YWHAZ | 0.342 | GAPDH | 0.634 | YWHAZ | 0.593 |
| HPRT | 0.344 | SDHA | 0.667 | ACTB | 0.596 |
| RPL19 | 0.361 | ACTB | 0.701 | G6PD | 0.666 |
| B2M | 0.362 | B2M | 0.720 | GAPDH | 0.681 |
| ACTB | 0.377 | G6PD | 0.740 | GYPC | 0.698 |
| GYPC | 0.412 | GYPC | 0.848 | RPL19 | 0.746 |
| TFRC | 0.455 | RPL19 | 1.000 | B2M | 0.760 |
| G6PD | 0.486 | HPRT | 1.188 | HPRT | 0.882 |
| PGK1 | 0.498 | PGK1 | 1.466 | TFRC | 1.158 |
| GAPDH | 0.579 | TFRC | 1.485 | PGK1 | 1.295 |