Table 2.
Candidate reference genes for normalization of qRT-PCR ranked according to their expression stability by the NormFinder applet.
| Control sheep (n = 18) | Disease-stressed sheep (n = 10) | Combined groups (n = 10 + 10) | |||
|---|---|---|---|---|---|
| Gene symbol | Stability value (ρ) | Gene symbol | Stability value (ρ) | Gene symbol | Stability value (ρ) |
| SDHA | 0.068 | YWHAZ | 0.046 | SDHA | 0.093 |
| YWHAZ | 0.125 | GAPDH | 0.064 | YWHAZ | 0.096 |
| HPRT | 0.132 | SDHA | 0.188 | ACTB | 0.099 |
| B2M | 0.146 | ACTB | 0.220 | GAPDH | 0.167 |
| RPL19 | 0.155 | B2M | 0.229 | RPL19 | 0.175 |
| ACTB | 0.174 | G6PD | 0.248 | G6PD | 0.184 |
| GYPC | 0.201 | GYPC | 0.397 | HPRT | 0.188 |
| TFRC | 0.245 | RPL19 | 0.540 | GYPC | 0.190 |
| G6PD | 0.271 | HPRT | 0.702 | B2M | 0.212 |
| PGK1 | 0.282 | PGK1 | 0.915 | TFRC | 0.274 |
| GAPDH | 0.354 | TFRC | 0.941 | PGK1 | 0.280 |