Table 3.
Details of primers and amplicons of the 11 candidate reference genes used for qRT-PCR analyses.
| Gene name | Primers sequences (forward/reverse) | Spanned exons | Amplicon size (bp) | PCR efficiency (%) | R2 |
|---|---|---|---|---|---|
| ACTB | CCAACCGTGAGAAGATGACC | 2nd | 97 | 102.1 | 0.999 |
| CCAGAGGCGTACAGGGACAG | 3th | ||||
| GYPC | ATCAACATCGCTGTCATTGC | 3th | 117 | 106.7 | 0.994 |
| CTCGTTGGTGTGGTATGTGC | 4th | ||||
| RPL19 | AGCCTGTGACTGTCCATTCC | 2nd | 126 | 102.0 | 0.998 |
| ACGTTACCTTCTCGGGCATT | 3th | ||||
| GAPDH | CTGGCCAAGGTCATCCAT | 7th | 86 | 104.1 | 0.997 |
| ACAGTCTTCTGGGTGGCAGT | 8th | ||||
| YWHAZ | AGACGGAAGGTGCTGAGAAA | 2nd | 123 | 100.0 | 0.998 |
| CGTTGGGGATCAAGAACTTT | 3th | ||||
| PGK1 | ACTCCTTGCAGCCAGTTGCT | 3th | 101 | 109.9 | 0.991 |
| AGCACAAGCCTTCTCCACTTCT | 4th | ||||
| HPRT | TTTATTCCTCATGGACTAATTATGGA | 2nd | 71 | 99.8 | 0.987 |
| CCACCCATCTCCTTCATCAC | 3th | ||||
| TFRC | TTCTGGGCAGACCTCAAATC | 4th | 106 | 100.9 | 0.991 |
| CAGCTTCACGTGGGACATAA | 5th | ||||
| SDHA | CATCCACTACATGACGGAGCA | 4th | 90 | 99.8 | 0.996 |
| ATCTTGCCATCTTCAGTTCTGCTA | 5th | ||||
| G6PD | TGACCTATGGCAACCGATACAA | 10th | 76 | 103.0 | 0.990 |
| CCGCAAAAGACATCCAGGAT | 11th | ||||
| B2M | CTGTCGCTGTCTGGACTGG | 1st | 86 | 98.9 | 0.997 |
| TTTGGCTTTCCATCTTCTGG | 2nd |