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. 2011 Nov 9;12(11):7748–7759. doi: 10.3390/ijms12117748

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Dilution series of two antibodies, HPA Ab 9 and HPA Ab 22. The antibodies were diluted 1:100, 1:1000, 1:10.000 and 1:100.000 and the signal from the expected positive antigen spot was measured. It was found that a linear relation between the intensity and the log of the concentration was found between dilutions 1:100 and 1:10.000. As no significant signals were found at dilutions 1:100.000, it is suggested that the sensitivity may reside at or lower than around 30 ng/mL for the lateral flow analysis of the two antibodies.