Figure 6.

Pharicin A induces mitotic arrest in leukemic cells resistant to paclitaxel. (A) Paclitaxel-resistant Jurkat cells were treated with paclitaxel (Taxol, 20 nM) or pharicin A (2 µM) for 24 h. The treated cells were stained by Wright-Giemsa dye (upper parts) or subjected to DNA content analysis for cell cycle distributions by flow cytometry (lower parts). (B) Paclitaxel-resistant Jurkat cells treated with paclitaxel (Taxol, 20 nM), pharicin A (2 µM) or vehicle for 24 h were stained with the antibody to p-H3S10. DNA was stained with DAPI. The experiment was repeated for three times. Representative images were shown. (C) U2OS cells and its subclone resistant to paclitaxel were treated with either pharicin A (2 µM) or paclitaxel (20 nM) or vehicle for 24 h. Treated cells were then processed for DNA content analysis using flow cytometry.