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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Nat Genet. 2011 Nov 6;43(12):1202–1209. doi: 10.1038/ng.990

Figure 4. Identification of novel Wnt targets with tumourigenic potential.

Figure 4

Potential regulators of Wnt signaling were identified by analysis with TRANSFAC 2009.3 (BIOBASE GmbH). The results were then cross-referenced with CIS corresponding to orthologs of genes identified from the CGH analysis of human sporadic colorectal cancers44 to generate a final set of CIS genes for further study. SW480 cells containing a stably integrated β-lactamase reporter gene under the control of the LEF/TCF binding consensus sequence were transiently transfected with siRNA (final concentration 20nM) and processed after 72 h. FRET read-out was measured using the manufacturer’s standard protocol. The fold change represented in the graph is calculated with respect to GC control. Error bars: s.d, n=4. Data were pooled from three independent experiments to determine statistical significance by Student’s t-test (***P<0.001, **P<0.01, *P<0.05).