Table 2. Characteristics of differentiated cultures of cloned RG cells.
Clone | Embryonic (E14.5) | Adult (P 50-75) | ||||||||
A2 | C4 | RGl-1 | HC_A | CTX_H | MES_D | SVZ_I | SVZ_K | SVZ_T | SVZ_M | |
neurons (IIIβ-tubulin immunreactive cells) | +(!) | +(!) | +(!) | + | + | +(!) | + | + | + | + |
astrocytes (GFAP immunreactive cells) | + | + | + | + | + | + | + | + | + | + |
oligodendrocyte precursors (O4- immunreactive cells) | + (!) | + | ni | + (!) | +(!) | + | + | + | + | + |
Gene expression | ||||||||||
vgat | ni | ni | + | + | + | + | + | + | + | + |
vglut1 | ni | ni | + | + | - | - | - | ni | ni | - |
vglut2 | ni | ni | + | - | - | - | - | ni | ni | - |
pitx3 | ni | ni | - | - | - | - | + | + | + | + |
tph2 | ni | ni | - | - | - | - | - | - | - | - |
dbh | - | - | - | - | - | - | - | - | - | - |
chat | ni | ni | - | - | - | - | - | - | - | - |
th | ni | ni | + | - | - | - | + | + | + | + |
ni: non investigated; (!): large frequency.