Figure 1. CFTR expression in cells obtained by nasal brushing. A.

Quantitative real-time PCR analysis for CFTR and β2-microglobulin in nasal cells obtained from two healthy donors. Calu-3 cells were used as positive control. B. Western blot analysis of CFTR in brushed nasal cells. CFTR protein was detected as a mature form (c) and an immature form (b) with anti-CFTR mAb 24.1. Anti-EGF receptor was used as control.