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. 2011 Dec 7;6(12):e28267. doi: 10.1371/journal.pone.0028267

Figure 4. Differential effects of cell division and microfilament inhibitors on expression of PKCδ and phosphorylated-PKCδ(Thr505) (labelled as P-PKCδ) in primary keratinocytes.

Figure 4

Primary mouse keratinocytes, after culturing for five days, were treated with the five inhibitors for 12 h, respectively. (A) Western blot analysis for expression of PKCδ (upper panel) and P-PKCδ (lower panel)after treatment with different inhibitors. Actin was examined as loading control. (B). Control and STS- and Lat-treated keratinocytes were triple-stained for spectrin (Green), PKCδ (Red) and nuclei (Blue). Scale bars are 15 µm. (C). Control and STS- and Lat-treated keratinocytes were triple-stained for spectrin (Green), P-PKCδ (Red) and nuclei (Blue). Arrows point to cells exhibiting strong phosphorylation of PKCδ(Thr505) and weak expression of spectrin. Scale bars are 20 µm.