Table 2. Comparison between mass spectrometry and western blot analyses.

	Mass Spectrometry		Western Blot
Protein	SC ratio	TMT ratio	Quantified	Ratio
	AAA vs. Control	AAA vs. Control	bands	AAA vs. Control	p-value
Extracellular superoxide dismutase	4.87	-	Unique1	1.94	0.007
Protein AMBP	1.12	-	Unique2	1.90	0.037
Gelsolin	0.81	-	Unique1	1.08	0.67
Heparin cofactor 2	0.76	-	Unique2	1.02	0.24
Carboxypeptidase N catalytic chain	0.71	-	Unique2	1.00	0.63
Alpha-2 antiplasmin	0.63	1.12	ND	NC	NC
Vinculin	0.44	-	Unique2	1.45	0.74
Filamin-A	0.40	-	ND	NC	NC
Alpha-actinin-1	0.20	-	ND	NC	NC
Talin-1	0.19	-	ND	NC	NC
Profilin-1	0.16	-	ND	NC	NC
Pleckstrin	0.12 ↓	-	Unique2	9.04	0.56
Adenylyl cyclase- associated protein 1	0.01	-	Unique2	0.89	0.90
Adiponectin	-	1.78	Both1	2.36	0.0092
			Upper1	1.87	0.033
			Lower1	1.69	0.036
Lysozyme C	-	1.39	ND	NC	NC
Carboxypeptidase B2	-	1.35	Unique1	1.45	0.0062
Kallistatin	-	0.89	Both1	1.32	0.11
			Upper1	1.20	0.47
			Lower1	1.31	0.046
Corticosteroid-binding globulin	-	0.72	Both1	1.04	0.25
			Upper1	1.00	0.23
			Lower1	1.10	0.74
Platelet basic protein	-	0.68	ND	NC	NC

SC: spectral count. TMT: tandem mass tag. Proteins verified by western blot analysis are indicated in italic. WB on crude plasma samples¹. WB on depleted plasma samples². ND: non detected. NC: non calculated.