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. 2011 Dec 7;6(12):e28643. doi: 10.1371/journal.pone.0028643

Figure 4. Lunar phase-dependency of SgCry mRNA expression in the brain and ovary.

Figure 4

Each Cry mRNA was calculated as a value relative to that of the Sgβ-actin gene. For each lunar phase, mRNA levels at ZT0, ZT6, ZT12 and ZT18 are averaged except for SgCry1 mRNA levels in the brain (mesencephalon and diencephalon). Since SgCry1 showed diurnal variation in the brain, values were normalized to reduce the deviation due to the daily rhythmic changes as follows; values at each daily time point (ZT0, ZT6, ZT12, and ZT18) were divided by the mean values of all the data for the same time point (0.0340, 0.0242, 0.0109, and 0.0141 for ZT0, ZT6, ZT12, and ZT18, respectively). Error bars represent ± SD. Lunar phases are indicated by schematic moon images. (A) Lunar changes in the SgCry1 mRNA levels in the brain. p<0.01, One-way ANOVA. Post hoc test; p<0.05, 5/25 vs 6/8, 6/15; p<0.01, 6/1 vs 6/8, 6/15, 6/22, 6/29, 7/7). (B) Lunar changes in the SgCry3 mRNA levels in the brain. p<0.01, One-way ANOVA. Post hoc test; p<0.05, 6/15 vs 6/22, 7/7; p<0.01, 5/25 vs 6/8, 6/15; p<0.01, 6/1 vs 6/8, 6/15, 6/22, 6/29, 7/7; p<0.01, 6/15 vs 6/29. (C, D) SgCry mRNA levels in the ovary plotted against lunar phase.