Table 4. The primers used to amplify the TRPA1 and TRPV1 cDNA fragments.
Target gene | Forward primer(5′→3′) | Reverse primer((5′→3′) |
TRPA1 | AF1: GCCTCGCAGGATRTCTTYAARGT | AR: CTTTSARSACYGTRTCCCAYTT |
AF2: CTYCGRAGYTTYATHAARAARAA | AR-1: TCTCCATCTGCTGCTTTCTG | |
AF2-1: TACTTGCCGACTTCGAAGCCTTA | ||
TRPV1 | VF1: GAYGAYCACACSGAYGGSGARGA | VR1: GGNACCAANGTYTTCCARTTYTT |
VF2: AARGAYATGGCTCCNATGGAYTC | VR2: CCSGGRTCYTCRTTGATDAT |