Figure 1.

NIRF induces G₁ arrest. (A) Rapidly growing HEK293 cells were transiently transfected with pEGFP-NIRF (pEGFP-C1-NIRF). After 48 h, the cells were harvested, and the cell cycle profiles were analyzed by FACS as described by Li et al.²⁰ The green fluorescent signal of EGFP was employed as the transfection marker, while PI staining was used to count the DNA contents of the cells. (B) The pEGFP-C1 empty vector was transfected and the cell cycle was analyzed as described for (A).