Table 3.

Performances of assembly programs with olive Roche-454 ESTs (minimum overlap: 40 bp, minimum overlap percent identity: 97%, maximum overhang: 30 bp)

		iAssembler	CAP3	MIRA	TGICL	Phrap	Newbler
No. unigenes		77,572	10,5103	127,565	80,540	70,489	69,301
Average unigene length (bp)		231.5	214.5	209.7	221	246.5	227.4
No. type I errors	identity < 97%	1	569	3	3,668	18,071	8,317
	overhang > 30 bp	1	11	2	1,621	5,066	11,266
No. type II errors		35	12,279	14,821	4,420	4,752	1,518
Total assembly errors		37	12,859	14,826	9,709	27,889	21,101
Run Time (minute)		227	79	57	101	43	7