Table 1.

PCR, sequencing and real time RT-PCR primers and cycling conditions used in this study

	Gene Name, Accession and SNP number	Primer /probe name and rs numbers	PCR Primer Sequence 5’→3’	Cycling conditions: denaturation, annealing and extension
			TaqMan Assay ID# or Assay sequences
PCR	PSIP1 AF 199339	LEDGF1 Seq5	F: TGG GCT CAA AGC ATTA ATC C R: TCT GTG GCG TAT ACA CAG TG	(95 °C, 10 mins), 35 cycles of (94 °C, 30 s; 60°C, 30 s; 72°C, 45 s) and (72 °C, 10 mins)
Sequencing		Seql Seq6	F: GCC AGA TATGAT TTA ATC TAG R: GTA GAC TTT TCC ATG ATT CCT GAC	(96 °C, 3 mins), 25 cycles of (96 °C, 15 s; 50°C, 15s; 60 °C, 2 s), (72 °C, 5 mins)
		Seq2 Seq5	F: GCC TGT ATA TAG AAA TAC TGG R: TCT GTG GCG TAT ACA CAG TG
		S9 R1	F: CTT CAA AGG ATA CAT GC R: GTA GAC TTT TCC ATG ATT CCT GAC
TaqMan Assay	SNP1	rs2277191	C_15883595_10
	SNP2	rs10283923	C_29529242_10
	SNP3	rs12339417	C_31936110_10
	SNP4	rs1033056	C_2757693_20
	SNP5	PSIP1_Q472L	PSIP1_Q472L_s AAAACCAAAGATCAAGGGAAGAAA Psip1_q472l_a TGTGAAATTGTTGGCTTTTTACCA
Real Time –RT PCR		PTZ1 PTZ2	F: GTC AAC CCC ACC GTG TTC TTC R: TTT CTG CTG TCT TTG GGA CCT TG	(95 °C, 6 s), (60 °C, 6 s) and (72 °C, 10s)
	GAPDH NM_002046	GAPDH1 GAPDH2	F: AAG GTC GGA GTC AAC GGA TT R: CTC CTG GAA GAT GGT GAT GG	(95 °C, 6 s), (60 °C, 6 s) and (72 °C, 10s)

2 F: → forward primer

3 R: → reverse primer

4 mins → minutes

5 s → seconds