Table 1.

Dose–response data for RDL with unnatural amino acids at positions 206 and 254

Mutant	Log EC50 (m)	EC50 (μm)	nH	n
WT	−4.909 ± 0.34	17	1.4 ± 0.2	7
F206A		>300		4
F206Y	−6.941 ± 0.09	0.11	2.2 ± 0.3	6
F206-FPhe	−4.190 ± 0.05	65	1.8 ± 0.3	5
F206-F2Phe	−3.257 ± 0.05	554	1.8 ± 0.3	6
F206-F3Phe	−2.990 ± 0.04	1022	2.0 ± 0.4	6
Y254A		NF		8
Y254F	−4.050 ± 0.05	76	2.1 ± 0.4	4
Y254-FPhe	−3.995 ± 0.07	110	1.3 ± 0.4	6
Y254F2-Phe	−2.135 ± 0.08	733	1.1 ± 0.2	4
Y254F3-Phe	−1.599 ± 0.07	2518	1.2 ± 0.3	7
F206Y/Y254F	−5.754 ± 0.07	1.8	1.9 ± 0.5	7
F146A	−4.140 ± 0.12	73	2.1 ± 0.2	5
F146F3Phe	−4.084 ± 0.15	82	1.4 ± 0.4	4

All values are expressed as mean and SEM.