Table 3.
Allosteric Patterns of the Native and Chimeric Enzymes
| nucleotide effectorsa | Sm | Ec | SM:rS5′ec |
|---|---|---|---|
| Pattern Common for Three Enzymes | |||
| ATP | +++b | ++ | + |
| UTP | 0 | 0 | 0 |
| CTP/ATP | ± | ± | ± |
| ATP/UTP | ± | ± | ± |
| Pattern Common for Native Ec and Chimera | |||
| CTP | +++ | – | – |
| ATP/CTP | 0 | ± | ± |
| CTP/UTP | ± | = | = |
| UTP/CTP | ± | = | = |
| Pattern Common for Native Sm and Chimera | |||
| UTP/ATP | ± | 0 | ± |
a
In the paired nucleotide studies, the first nucleotide listed was used at increasing concentration in the presence of a certain concentration of the second listed nucleotide. For example, ATP/CTP indicates varying concentrations of ATP in the presence of a saturating concentration of CTP; CTP/ATP indicates varying concentrations of CTP in the presence of a saturating concentration of ATP.
b
+++ represents strong activation; ++, activation; +, weak activation; 0, no effect; –, inhibition; =, synergistic inhibition; ±, competition.