Table 1.
Primers (IDT, Coralville, IA)
| Gene | Forward Primer | Reverse Primer | Temperature, °C |
|---|---|---|---|
| β3-AR | 5′-CAG-AAC-TCA-CCG-CTC-AAC-AG | 5′-CCT-TCA-TGT-GGG-AAA-TGG-AC | 57.1 |
| AgRP | 5′-TTC-CCA-GAG-TTC-TCA-GGT-CTA | 5′-ATC-TAG-CAC-CTC-TGC-CAA-A | 55.0 |
| AGT | 5′-CTG-GGC-AAG-ATG-GGT-GAC | 5′-CTG-GCC-TGC-TTG-GAG-TTC | 55.8 |
| FAS | 5′-ATA-TGA-AGG-TGG-TGG-AGG-TG | 5′-TGC-AGC-TTG-GTC-TGA-ACA-TC | 55.8 |
| L32 | 5′-CAG-ACG-CAC-CAT-CGA-AGT-TA | 5′-AGC-CAC-AAA-GGA-CGT-GTT-TC | 61.2 |
| LPL | 5′-ACA-CTG-GAA-ACG-CTG-TTG-TG | 5′-TTC-CGG-ATA-AAA-CGT-TCT-CG | 55.8 |
| NPY | 5′-CTC-TGC-GAC-ACT-ACA-TCA-A | 5′-GGG-GCA-TTT-TCT-GTG-CTT-T | 61.2 |
| POMC | 5′-TCC-ATA-GAC-GTG-TGG-AGC-TG | 5′-ACT-TCC-GGG-GAT-TTT-CAG-TC | 57.1 |
| UCP1 | 5′-GTG-TAC-CCA-GCT-GTG-CAA-TGA | 5′-CGC-AAA-CCC-TTT-GAA-AAA-GG | 61.2 |
β3-AR, β3-adrenergic receptor; AgRP, agouti-related peptide; AGT, angiotensinogen; FAS, fatty acid synthase; LPL, lipoprotein lipase; NPY, neuropeptide Y; POMC, proopiomelanocortin; UCP1, uncoupling protein 1.