Table 5.

Hepatic and hypothalamic gene expression in 2nd-pregnancy offspring

Pregnancy no. 2	LF	HF	DR2
Liver	(n = 17–18)	(n = 28–32)	(n = 14)
Il1b	1.000 ± 0.229	2.583 ± 0.592*	1.395 ± 0.295
Il6	1.000 ± 0.138	1.538 ± 0.212	1.233 ± 0.280
Tnf	1.000 ± 0.124	0.924 ± 0.061	0.938 ± 0.081
Il1r1	1.000 ± 0.090	1.006 ± 0.093	0.638 ± 0.045*,#
Il10	1.000 ± 0.121	3.335 ± 0.674*	1.726 ± 0.402
Ccl2	1.000 ± 0.170	0.620 ± 0.083*	0.854 ± 0.134
Crp	1.000 ± 0.078	1.142 ± 0.095	1.224 ± 0.140
Fasn	1.000 ± 0.118	1.813 ± 0.190*	2.644 ± 0.525**
Srebf1	1.000 ± 0.062	1.254 ± 0.068*	1.061 ± 0.085
Ppara	1.000 ± 0.109	1.036 ± 0.090	0.638 ± 0.099*,##
Pck1
Mttp	1.000 ± 0.073	0.911 ± 0.060	0.892 ± 0.087
Fas	1.000 ± 0.107	0.847 ± 0.057	0.985 ± 0.133
Bax	1.000 ± 0.069	1.006 ± 0.050	0.861 ± 0.045
Bcl2	1.000 ± 0.046	1.102 ± 0.046	0.978 ± 0.055
Hypothalamus	(n = 14–15)	(n = 8–10)	(n = 14)
Hcrt	1.000 ± 0.071	1.173 ± 0.077	1.010 ± 0.078
Pmch	1.000 ± 0.042	1.065 ± 0.061	0.923 ± 0.075
Il1b	1.000 ± 0.142	1.603 ± 0.289	1.125 ± 0.188
Il6	1.000 ± 0.090	1.337 ± 0.137	0.994 ± 0.164

Values are means ± SE. Gene expression was normalized to β-actin.

^*P < 0.05,

^**P < 0.01 vs. LF pups;

^#P < 0.05,

^##P < 0.01 vs. HF pups.