Fig. 1.

Enterocyte adhesion and spreading on laminin is increased by CXCL12 stimulation. An intestinal epithelial cell line, IEC-6, cells were seeded to laminin, collagen IV, or BSA-coated wells. A: IEC-6 cell adhesion was increased on laminin and collagen IV compared with control wells. Stimulation with CXCL12 (2.5 nM) (solid bars) increased adhesion compared with unstimulated control cells (open bars). CXCL12 increases adhesion on laminin but had little to no effect on adhesion to BSA or collagen IV. B: adherent IEC-6 cells spread more on laminin coating compared with collagen IV. CXCL12 stimulation (2.5 nM) (solid bars) increased the area of IEC-6 cells spreading on laminin similar to the positive control epidermal growth factor (EGF) (50 ng/ml) (hatched bars). CXCL12-induced spreading was specific for laminin and was not seen on collagen IV. C: representative immunofluorescence images of calcein-AM-loaded IEC-6 cells adhering to laminin or BSA. D: representative photomicrographs of spreading IEC-6 cells on laminin dishes. Data are mean number of adherent cells or cell area ± SE from 3–5 experiments. Asterisk denotes statistically significant difference from untreated cells or BSA (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001) measured by two-way ANOVA. Scale bar = 100 μm in C and 50 μm in D.