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. 2011 Sep 15;301(6):G968–G980. doi: 10.1152/ajpgi.00208.2011

Fig. 2.

Fig. 2.

CXCL12-induced adhesion and spreading are dependent on intracellular calcium. IEC-6 cells were pretreated with 10 μM BAPTA-AM (shaded bars) or PBS (open, solid, and hatched bars) for 30 min. A: cell adhesion to laminin was increased by stimulation with 2.5 nM CXCL12 (solid bars) compared with untreated cells (open bars). Adhesion elicited by CXCL12 stimulation was blocked by BAPTA-AM pretreatment. B: CXCL12 (2.5 nM) (solid bar) and EGF (50 ng/ml) (hatched bar) increased spreading of IEC-6 cells cultured on laminin for 45 min. Chelation of intracellular calcium specifically blocked CXCL12-stimulated increase in cell area while having little effect on EGF-induced spreading. Values are means ± SE of from 3–4 separate experiments. Asterisk denotes statistically significant difference from untreated cells or BSA (**P ≤ 0.01). Pound symbol indicates statistically significant difference between CXCL12-stimulated cells pretreated with PBS or BAPTA-AM (##P ≤ 0.01). Dagger indicates a statistically significant difference (†P ≤ 0.05) between unstimulated and EGF-treated BAPTA-AM pretreated cells.