Fig. 8.

α6-Integrin is required for CXCL12-stimulated migration. A: representative immunoblot analyses of WT, pLKO-scrambled and pLKO-shα6 integrin IEC-6 cells showed decreased α6, whereas level of other integrins was unaffected. B: quantification of α6 reduction in WT, pLKO-scrambled, pLKO-shα6 IEC-6 cells. Values are relative α6 expression normalized to GAPDH loading control compared with WT cells from 6 different immunoblot analyses. C: CXCL12 stimulation (2.5 nM) (solid bars) was unable to stimulate increased migration in α6-depleted IEC-6 cells, whereas 5 ng/ml TGF-β1 (hatched bars) stimulated significantly more migration than untreated cells (open bars). Inducible migration within WT and pLKO-scrambled control cells remained intact. Values are means ± SE of 6 individual experiments. Asterisk denotes statistically significant difference from WT or untreated cells (*P ≤ 0.05, **P ≤ 0.01). Scale bar = 125 μm.