Skip to main content
. 2011 Aug 17;301(6):C1325–C1343. doi: 10.1152/ajpcell.00054.2011

Fig. 4.

Fig. 4.

Surface expression of human RhAG-HA or RhAG-HA F65S in Xenopus oocytes is not increased by coexpression of glycophorin B (GPB). A: anti-HA immunoblot of lysates from oocytes expressing HA-tagged wild-type, F65S, or F65V mutant polypeptides. Each lane was loaded with lysate of particulate fraction prepared from 10 oocytes. One of two blots with similar results is shown. B–F: confocal immunofluorescence images of median intensity HA antigen surface expression by Xenopus oocytes expressing COOH-terminally HA-tagged wild-type RhAG (B and C) or RhAG mutant F65S (10 ng cRNA; D and E) in the presence (C and E) or absence (B and D) of coexpressed GPB (10 ng cRNA). A representative water-injected oocyte us shown in F. G: surface expression of wild-type RhAG-HA and RhAG-HA F65S measured as mean relative fluorescence intensities. Values are means ± SE for (n) oocytes, and did not differ one from another (Tukey-Kramer analysis and Dunnett's t-test).