Fig. 6.

Human RhAG F65S mediates increased ouabain-resistant ⁸⁶Rb⁺ uptake compared with wild-type RhAG. A: ⁸⁶Rb⁺ uptake by oocytes previously injected with water or with cRNA encoding wild-type RhAG or RhAG F65S (10 ng cRNA). *P < 0.05 (gray bar) compared with wild-type RhAG (Dunnett's t-test). B: ⁸⁶Rb⁺ uptake by oocytes expressing wild-type RhAG was enhanced by coexpressed GPB (*P < 0.05, Dunnett's t-test). Rates were water-subtracted. C: coexpression of wild-type RhAG with wild-type AE1 did not increase RhAG-mediated ⁸⁶Rb⁺ uptake at low (1 ng) or higher cRNA concentrations (10 ng cRNA). Rates were water-subtracted. Ouabain (0.5 mM) and bumetanide (5 μM) bumetanide were present in all experiments. *P < 0.05 compared with wild-type RhAG (Dunnett's t-test).