TM4SF10 colocalizes with ADAP and Fyn in podocytes. Detection by immunohistochemistry of TM4SF10-GFP (B,H) or endogenous TM4SF10 (E) in podocytes and colocalization with endogenous ADAP (A,D) or endogenous Fyn (G); respective merged images in C, F, and I. TM4SF10 was originally described as an endoplasmic reticulum (ER) and plasma membrane protein (4), and both endogenous and GFP-tagged TM4SF10 localized to intracellular compartments and the plasma membrane, with concentrations at cell-cell contacts (arrow).