Fig. 3.

TM4SF10 coimmunoprecipitates with ADAP but not with Fyn in podocytes. A: ADAP interactions with Fyn and TM4SF10 were confirmed in podocytes stably expressing TM4SF10-GFP (“TM4-GFP”) by immunoprecipitating with Fyn, ADAP, or GFP antibodies and Western blotted for ADAP (“input”, whole cell lysates). B: Fyn interactions with TM4SF10 were tested in similar immunoprecipitation experiments, using podocytes stably expressing TM4SF10-GFP (TM4-GFP) or GFP (as a negative control), after transient transfection with Fyn plasmid. Cell lysates were immunoprecipitated with either: a control species and isotype matched IgG (IgG); an anti-Fyn antibody (Fyn); or an anti-GFP antibody (GFP), and Western blotted for either Fyn or GFP. Some of the IgG heavy chain of the immunoprecipitating antibody is observed in these blots due to cross reactivity with the Western blotting antibody.