Fig. 6.

Puromycin aminonucleoside (PAN) treatment induces reexpression of TM4SF10. A: cultured podocytes were treated with PAN or phosphate-buffered saline as a control and were immunostained for native expression of TM4SF10 (green) and cadherin (red), along with the nuclear stain DAPI (blue). Note the simplification of interdigitations of cell-cell contacts (cadherin-positive cell junctions) with PAN treatment compared with the control treated cells. B: immunohistochemistry for TM4SF10 (green) in kidney sections from rats treated with PAN or saline as a control using the standard PAN nephrosis model of podocyte injury. In this model, postinjection day 2 is a time point before the onset of severe proteinuria, whereas day 10 is a time point where rats exhibit nephrotic range proteinuria. Panels are matched exposures; ×40 magnification.