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ANG II induces JNK phosphorylation maximally at 30 min. Vascular smooth muscles cells (VSMCs) were stimulated with 100 nM ANG II for the indicated times, and Western blotting with anti-phospho (p)-JNK antibody or total JNK was performed. A representative blot is shown at top (n = 3). IB, immunoblotting. Bar graph represents means ± SE. *P < 0.05 compared with no stimulation.
