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Inhibition of JNK does not prevent ANG II-induced IRS-1 degradation. VSMCs were treated with ANG II (100 nM) for 18 h with or without SP600125 (30 nM), and samples were immunoblotted for IRS-1. Bar graph represents loss of IRS-1 protein following ANG II treatment, expressed as means ± SE of percent decrease from control (n = 3).
