Fig. 5.

Effects of small interfering (si)RNA knockdown of the sGC β₁-subunit in BPAs on the response to H₂O₂ relaxation and VASP phosphorylation. A: Western blot analysis showing that sGC-β₁ siRNA transfection for 48 h decreased expression of the sGC β₁-subunit (n = 12). B: with sGC-β₁ siRNA transfection, relaxation to H₂O₂ was attenuated (n = 12) and VASP phosphorylation (C) was decreased (n = 11) compared with the scrambled siRNA control. Decreases in force associated with the relaxation to peroxide were not reported as percent relaxation because sGC depletion caused an increase in the contraction to 25 mM KCl (see Fig. 6A). *P < 0.05 vs. Ctrl.