Fig. 9.

Effects of acute treatment of freshly isolated BPAs with the heme oxidant/sGC inhibitor 10 μM ODQ on force generation to K⁺ and on the contraction to hypoxia and effects of spermine NONOate on PKG dimerization under hypoxia. A and B: summary data showing that 10 μM ODQ did not alter the force generation to K⁺ under normoxia normalized to 30 mM K⁺ (30K) force (n = 8; A) or the contraction to hypoxia normalized to 30K force (n = 8; B). C: Western blot analysis showing that disulfide-mediated dimerization in the presence of 1 μM spermine NONOate was not altered (top) and summary data showing that spermine NONOate had no effects on the disulfide-mediated dimerization of PKG (bottom; n = 8).