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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Neurochem. 2011 Nov 3;119(6):1294–1305. doi: 10.1111/j.1471-4159.2011.07517.x

Table 1.

Cytoplasmic loop of Catsup interacts physically with TH and GTPCH in a yeast two hybrid assay1

Catsup Loop2 TH Fragments3 SD(-T,-W,-H) LacZ
Full-length Full-length + +
C-terminal domain Full-length + +
Full-length N-terminal half + +
C-terminal domain N-terminal half + +
Full-length C-terminal half − −
C-terminal domain C-terminal half − −
N-terminal domain Full-length − −
Catsup Loop 1 GTPCH Fragments 4 SD(-T,-W,-H) LacZ
C-terminal domain Full-length GTPCHa/Pu-RA + +
C-terminal domain N-terminal GTPCHa/Pu-RA + +
N-terminal domain Full-length GTPCHa/Pu-RA − −
C-terminal domain Full-length GTPCHc/Pu-RC + +
C-terminal domain N-terminal GTPCHc/Pu-RC + +
N-terminal domain Full-length GTPCHc/Pu-RC − −
C-terminal domain GTPCH catalytic domain − −
1

The full length Catsup cytoplasmic loop and its C-terminal and N-terminal halves in the pGAD-C1 vector were used as bait; prey constructs were the full length, N-terminal and C-terminal fragments of TH, and the full length protein and N-terminal regulatory and catalytic domain fragments of GTPCH isoforms RA and RC in the pGBD-C1 vector. (+): yeast cells grew on SD/-Trp-Ade-His plates and developed blue coloration after incubation with X-gal. (−): no growth on the selection medium.

2

Full length Catsup loop: residues 242-363; N-terminal fragment: residues 242- 295; C-terminal fragment: residues 293-363.

3

Full length TH: residues 1-508; N-terminal half: residues 1-269; C-terminal half: residues 269-508.

4

Full length GTPCHa/RA: residues 1-324; N-terminal GTPCHa/RA: residues 1-117. Full length GTPCHc/RC: residues 1-308; N-terminal GTPCHc/RC: residues 1-101. Catalytic domain (shared by GTPCH-RA and –RC): residues 118 in RA and 102 in RC to C- terminus.