Fig. 2.

RSG attenuates HYP-induced increases in ET-1 signaling pathway mRNA levels in mouse lung. C57BL/6 mice were exposed to NOR (21% O₂) or HYP (10% O₂) ± treatment with RSG as described in Fig. 1. Each bar represents the mean ± SE ET-1 (A), endothelin-converting enzyme (ECE)-1 (B), endothelin receptor A (ETAR, C), and endothelin receptor B (ETBR, D) mRNA relative to ribosomal 9S and expressed relative to control. P < 0.05 vs. NOR (*) and vs. HYP (+); n = 5. Mouse lungs were collected for Western blotting using antibodies directed against ECE-1, ET-1, ETBR, ETAR, and CDK4 (E). Representative blots from 3 separate experiments are presented above average densitometric values ± SE for each protein. P < 0.05 vs. NOR (*) and vs. HYP (+); n = 3.