Fig. 3.

RSG attenuates HYP-induced increases in HPAEC ET-1 signaling components and proliferation. HPAEC were exposed to NOR (21% O₂) or HYP (1% O₂) for 72 h. Selected cells were treated during the final 24 h of exposure with RSG (10 μM). Each bar represents the mean ± SE ET-1 (A), ECE-1 (B), and ETBR (C) mRNA levels relative to ribosomal 9S normalized to the control value from 6–9 samples in each treatment group. P < 0.05 vs. NOR (*) and vs. HYP (+). HPAECs were collected for Western blotting using antibodies directed against ECE-1, ET-1, ETBR, and CDK4 (D). Representative blots from 3 separate experiments are presented above average densitometric values ± SE for each protein. P < 0.05 vs. NOR (*) and vs. HYP (+); n = 3. The impact of HYP ± RSG on HPAEC proliferation was determined using cell counting and MTT assays (E). Each bar represents the mean ± SE relative to control. P < 0.05 vs. NOR (*) and vs. HYP (+); n = 6.