Table 1.
Primer information for real-time quantitative PCR
| Gene Symbol | mRNA Product Length, bp | Annealing Temperature, °C | Primer Sequences (5′-3′) | GenBank Accession No. |
|---|---|---|---|---|
| Human | ||||
| ET-1 | 359–418 | 60 | F: TCTCTGCTGTTTGTGGCTTG | NM_001955 |
| (60 bp) | R: GAGCTCAGCGCCTAAGACTG | |||
| ECE-1 | 552–624 | 60 | F: CATCAAGCACCTCCTCGAA | NM_001397 |
| (73 bp) | R: ACGGTAGTATACTTGCGCCTTT | |||
| ETAR | 1,222–1,287 | 60 | F: GCGCTCTTAGTGTTGACAGGT | NM_001957 |
| (66 bp) | R: GAATCCCAATTCCCTGAACA | |||
| ETBR | 158–240 | 60 | F: CTTGGAGTCTGGACATCTGAAA | NM_000115 |
| (83 bp) | R: CTGCATGCTGCTACCTGCT | |||
| 9S rRNA | 237–338 | 60 | F: CTGACGCTTGATGAGAAGGAC | NM_001013 |
| (102 bp) | R: CAGCTTCATCTTGCCCTCAT | |||
| Mouse | ||||
| ET-1 | 447–531 | 60 | F: CTGCTGTTCGTGACTTTCCA | NM_010104 |
| (85 bp) | R: TCTGCACTCCATTCTCAGCTC | |||
| ECE-1 | 773–846 | 60 | F: CCCTGATGGAGCTGATTGA | NM_199307 |
| (74 bp) | R: GTCCTGGAAGTTGTCCTTGG | |||
| ETAR | 919–979 | 60 | F: GGGCATCACCGTCTTGAA | NM_010332 |
| (61 bp) | R: GGAAGCCACTGCTCTGTACC | |||
| ETBR | 1,135–1,226 | 60 | F: CGGTATGCAGATTGCTTTGA | NM_007904 |
| (92 bp) | R: AACAGAGAGCAAACACGAGGA | |||
| 9S rRNA | 437–552 | 60 | F: ATCCGCCAACGTCACATT | NM_029767 |
| (116 bp) | R: CCGCCGCCATAAGGAGAAC |
ET-1, endothelin-1; ECE-1, endothelin-converting enzyme-1; ETAR, endothelin receptor A; ETBR, endothelin receptor B; F, forward; R, reverse.