Fig. 3.

HIF-1 activation by the prolyl hydroxylase inhibitor dimethyloxalylglycine (DMOG) protects against airway epithelial barrier loss by H₂O₂. Monolayers of 16HBE14o− cells seeded on Transwell inserts were treated with DMOG (1 mM) for 24 h and subsequently challenged with 500 μM H₂O₂, and epithelial barrier function was monitored by measuring changes in TER (A) or permeability to FITC-dextran (B). Effects of DMOG and/or H₂O₂ on HIF-1α accumulation were determined by Western blot (C). P < 0.05 compared with control (*) and with H₂O₂ (#).