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. 2011 Sep 9;301(6):L927–L936. doi: 10.1152/ajplung.00049.2011

Fig. 3.

Fig. 3.

Increased levels of CCL11 and CCL24 RNA in macrophages from OVA-immunized and -challenged APN−/− and WT mice. A: representative flow sorting method for isolation of pulmonary macrophages. Last panel shows a cytospin of the isolated cells. B: levels of RNA encoding CCL11, CCL24, and CCL2 in lung macrophages isolated from the lungs of WT and APN−/− mice following OVA immunization and OVA or PBS challenge (n = 7–8 mice/group, P < 0.05 by 2-way ANOVA for genotype and OVA vs. PBS, *P < 0.05 by t-test). C: levels of RNA encoding arginase-1, YM1, and inducible nitric oxide synthase (iNOS) in lung macrophages isolated from the lungs of WT and APN−/− mice following OVA immunization and OVA or PBS challenge (n = 7–8 mice/group, P < 0.05 by 2-way ANOVA for OVA vs. PBS but not for genotype, *P < 0.05 by t-test). D: levels of phosphorylated signal transducer and activator of transcription (STAT) 6 in RAW 264.7 macrophages following pretreatment with APN (10 μg/ml) and IL-4 (100 ng/ml) (each condition was done in triplicate, *P < 0.05 by t-test). No Stim, no stimulation.